Table 2

Possible new annotations for C. thermocellum ORFs based on identified metabolic gaps

Missing EC number

Enzyme name

Possible Cth ORF

Current annotation

Reciprocal best hita

E valueb


2.7.1.107

diacylglycerol kinase

Cthe_3168

hypothetical protein

A0R923

7.00E-35


3.2.1.52

Hexosaminidase

Cthe_0787

isoleucyl-tRNA synthetase

A4N847

0


2.7.7.39

CDP-glycerol pyrophosphorylase

Cthe_1276

pantetheine-phosphate adenylyltransferase

A1SHB9

3.00E-07


2.7.8.8

phosphatidylserine synthase

Cthe_0158

Ribonuclease

A9JBA9

1.00E-68


4.1.1.65

phosphatidylserine decarboxylase

Cthe_0505

formate acetyltransferase

A4NBN7

0


1.3.99.1

succinate dehydrogenase

Cthe_2355

L-aspartate oxidase

Q97W79

1.00E-94


1.3.1.6

NADH-fumarate reductase

Cthe_2355

L-aspartate oxidase

B0VG44

5.00E-98


1.3.5.1

succinate dehydrogenase

Cthe_2355

L-aspartate oxidase

A4YEK0

2.00E-85


6.2.1.5

succinate--CoA ligase

Cthe_1907

amino acid adenylation domain

A3P3B3

3.00E-40


6.4.1.2

acetyl-CoA carboxylase

Cthe_0699

carboxyl transferase

A0RY61

8.00E-169


6.3.4.14

biotin carboxylase

Cthe_0949

carbamoyl-phosphate synthase, large subunit

B2J980

0


4.1.3.38

aminodeoxychorismate lyase

Cthe_0026

queuosine biosynthesis protein

Q03L66

3.00E-54


3.1.3.1

alkaline phosphatase

Cthe_2965

binding-protein-dependent transport systems inner membrane component

B0USD4

1.00E-59


2.6.1.2

alanine transaminase

Cthe_0755

aminotransferase, class I and II

Q7LYW0

3.00E-66


2.6.1.51

serine--pyruvate transaminase

Cthe_0265

aminotransferase, class V

B4BE13

0


2.7.1.39

homoserine kinase

Cthe_0397

ABC transporter related protein

A5 M0U7

3.00E-140


3.1.3.3

phosphoserine phosphatase

Cthe_0256

histidine kinase

A9G173

3.00E-54


2.7.1.40

pyruvate kinase

Cthe_1955

RNA binding S1

A5LC67

0


1.2.2.1

formate dehydrogenase

Cthe_0199

4Fe-4S ferredoxin, iron-sulfur binding

Q2LVY6

9.00E-11


1.7.99.4

nitrate reductase

Cthe_0200

FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Q11VH4

4.00E-24


2.2.1.2

transaldolase

Cthe_0217

Glucose-6-phosphate isomerase

Q2S6E8

1.00E-22


6.3.4.1

GMP synthase

Cthe_0375

GMP synthase, large subunit

A2C5P2

4.00E-176


1.2.1.2

formate dehydrogenase

Cthe_0341

NADH dehydrogenase (quinone)

B5IPC7

6.00E-126


5.3.3.2

isopentenyl-diphosphate Delta-isomerase

Cthe_1022

Glycerol-3-phosphate dehydrogenase

A8VXT5

2.00E-94


2.5.1.29

farnesyltranstransferase

Cthe_0831

Polyprenyl synthetase

B2J443

4.00E-69


2.5.1.33

trans-pentaprenyltranstransferase

Cthe_0564

Trans-hexaprenyltranstransferase

Q6KZR8

3.00E-25


3.2.1.108

lactase

Cthe_0212

Beta-glucosidase

P09848

3.00E-89


3.5.1.19

nicotinamidase

Cthe_1178

isochorismatase hydrolase

Q6F6U3

6.00E-08


1.2.4.4

branched chain keto acid dehydrogenase

Cthe_0547

periplasmic solute binding protein

A8VXE7

2.00E-27


aUniProt accession numbers

bE value based on reciprocal best hit against C. thermocellum gene

Roberts et al. BMC Systems Biology 2010 4:31   doi:10.1186/1752-0509-4-31

Open Data