Reproducibility of instability quantification methods. Instability indices were determined from 100 ng genomic DNA isolated from 17 tissues of 2-6 HdhQ111/+ mice at 5 months of age. (A) The table shows replicate number of mice for each tissue (n) and numbers of samples where the highest peak was shifted from the main allele (tail's highest peak). Zero indicates no major allele shift and +1 indicates one CAG unit increase. B. Instability index. (C, D, E) The relative peak height correction method was applied to different methods of quantification such as: a contraction and expansion index (C), the number of contracted/expanded peaks (D) and the relative composition of contracted/unchanged/expanded peaks (E). Data bars represent mean ± SE. TL, tail; CTX, cortex; ST, striatum; CB, cerebellum; LV, liver; HT, heart; LN, lung; STO, stomach; SP, spleen, SKN, skin; KD, kidney; OV, ovary; TS, testis; HC, hippocampus; PAN, pancreas; LI, large intestine; and SI, small intestine.
Lee et al. BMC Systems Biology 2010 4:29 doi:10.1186/1752-0509-4-29