Additional file 17.

Overview of pathway specific consensus reactions for different time points of intraerythrocytic cycle. Flux distributions have been predicted with our improved flux balance approach (see Figure 1) for each time point of the intraerythrocytic developmental cycle for which a gene expression profile exists. Simulations were conducted on the basis of the combined metabolic network of parasite and host and additional constraints reflecting knowledge about the blood stage. Furthermore, the expression status of genes during preceding time points was considered for the flux calculations. Consensus reactions, which are reactions that are predicted to be active for all gene expression samples covering the same stage, were determined. These reactions are more likely to actually occur during a certain stage, since they are derived from different data samples. In order to identify consensus reactions we grouped all calculated flux profiles corresponding to the blood stage into seven sets (eRing, lRing, eTropho, lTropho, eSchiz, lSchiz, Mero; see Additional file 3) with respect to represented stages and determined those reactions that carry a non-zero flux in all flux profiles of the same set. For each blood stage and each metabolic pathway the fraction of consensus reactions per total number of pathway reactions was computed to uncover the distribution of consensus reactions among pathways. To get an impression of how many consensus reactions are shared between the different stages, this fraction was also computed for those consensus reactions that two sets have in common. The darker the color of a matrix entry the lower is the percentage of consensus reactions.

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Huthmacher et al. BMC Systems Biology 2010 4:120   doi:10.1186/1752-0509-4-120