Additional file 13.

Reaction distribution among stage-specific fluxes. Flux distributions have been predicted with our improved flux balance approach (see Figure 1) for each time point of the intraerythrocytic developmental cycle for which a gene expression profile exists. Simulations were conducted on the basis of the combined metabolic network of parasite and host and additional constraints reflecting knowledge about the blood stage. Furthermore, the expression status of genes during preceding time points was considered for the flux calculations. Subsequently, it was counted in how many of these stage-specific flux distributions a particular reaction was carrying a non-zero flux. The resulting histogram is shown here. The x-axis gives the number of flux distributions within which a reaction carries a non-zero flux and the y-axis indicates the frequency. In other words, the left most bar of the histogram represents the number of reactions that exclusively occur in a single flux distribution and are therefore very stage-specific, while the right most bar represents the number of reactions that are present in all (96) flux distributions related to the blood stage.

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Huthmacher et al. BMC Systems Biology 2010 4:120   doi:10.1186/1752-0509-4-120