Open Access Research article

Mechanism of PP2A-mediated IKKβ dephosphorylation: a systems biological approach

Johannes Witt1*, Sandra Barisic2, Eva Schumann2, Frank Allgöwer3, Oliver Sawodny1, Thomas Sauter14 and Dagmar Kulms2*

Author Affiliations

1 Institute for System Dynamics, Universität Stuttgart, Pfaffenwaldring 9, 70569 Stuttgart, Germany

2 Institute of Cell Biology and Immunology, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany

3 Institute for Systems Theory and Automatic Control, Universität Stuttgart, Pfaffenwaldring 9, 70569 Stuttgart, Germany

4 Life Sciences Research Unit, University of Luxembourg, 162 A, avenue de la Faïencerie, 1511 Luxembourg, Luxembourg

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BMC Systems Biology 2009, 3:71  doi:10.1186/1752-0509-3-71

Published: 16 July 2009

Additional files

Additional file 1:

Supplementary information about the modelling procedure. Includes a more detailed description of several aspects of the modelling procedure.

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Additional file 2:

Simulation results of the reference model with different parameterization. The fit is slightly worse but visually more satisfactory than the reference scenario.

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Additional file 3:

Simulation results for the alternative model with delayed PP2A activation. Shows the fit and describes the model equations of the alternative model.

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Additional file 4:

Representative Western Blot Analysis. Representative Western Blot of PP2Ac-dependent IKKβ phosphorylation

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Additional file 5:

Simulation results for IL-1 receptor internalisation. Shows the amount of total IL-1 receptor (ILR + ILRc) in the reference scenario.

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