Schematic of sampling and scoring analysis to determine intracellular flux changes. (A) Reaction fluxes are sampled for two conditions. (B & C) Sample of flux differences is calculated by selecting random flux values from each condition to obtain a distribution of flux differences for each reaction. (D) Standardized reaction Z-scores are determined, which represent how far the sampled flux differences deviates from a zero flux change. Reaction scores can be used in visualizing perturbation subnetworks and analyzing reporter metabolites and subsystems.
Mo et al. BMC Systems Biology 2009 3:37 doi:10.1186/1752-0509-3-37