|
VEGF concentration and receptor densities for the tumor (breast cancer) |
||||
| Measured parameter |
Tissue model |
|||
| Value |
Unit |
Value |
Unit |
|
|
|
||||
| Free VEGF concentration |
||||
| Breast cancer |
0.5–1.5 |
pM |
2.6–7.83 10-4 |
pmol/cm3 tissue |
|
|
||||
| Total tumor VEGF |
||||
| Breast cancer |
13 |
pg/mg tumor |
0.3 |
pmol/cm3 tissue |
|
|
||||
| VEGFR1 tissue concentration |
||||
| 102–105 |
#/EC |
1.7 10-3–1.7 100 |
pmol/cm3 tissue |
|
|
|
||||
| VEGFR2 tissue concentration |
||||
| 104 |
#/EC |
1.7 10-1 |
pmol/cm3 tissue |
|
|
|
||||
| NRP1 tissue concentration |
||||
| 103–106 |
#/EC |
1.7 10-2-1.7 101 |
pmol/cm3 tissue |
|
|
|
||||
| ECM binding site density |
||||
| ECM |
0.75 |
μM |
389 |
pmol/cm3 tissue |
| Vessel BM |
13 |
μM |
4 |
pmol/cm3 tissue |
| Tumor cell BM |
13 |
μM |
32 |
pmol/cm3 tissue |
|
The conversion of receptor densities to tissue concentrations is based on the relationship mentioned in table 5, and the surface area of an endothelial cell, 1000 μm2. VEGF concentration is normalized based on the entire interstitial space, since it diffuses throughout: 5.2 108 (pmol/cm3 tissue)/M (here, M = moles/liter interstitial space). VEGF binding sites in the ECM and BMs are based on those volumes: 5.2 108 (pmol/cm3 tissue)/M(ECM), 2.7 105 (pmol/cm3 tissue)/M(EBM), 2.5 106 (pmol/cm3 tissue)/M(PBM) [4]. | ||||
Stefanini et al. BMC Systems Biology 2008 2:77 doi:10.1186/1752-0509-2-77 |
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