|
VEGF concentration and receptor densities for the healthy tissue (human vastus lateralis) |
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| Measured parameter |
Tissue model |
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| Value |
Unit |
Value |
Unit |
|
|
|
||||
| Free VEGF concentration |
||||
| Human vastus lateralis, rest |
1 |
pM |
6.2 10-5 |
pmol/cm3 tissue |
|
|
||||
| Total VEGF tissue concentration |
||||
| Human vastus lateralis, rest |
1–2 |
pg/μg protein |
3.4–6.9 |
pmol/cm3 tissue |
|
|
||||
| VEGFR1 tissue concentration |
||||
| Human vastus lateralis, rest |
1.6–1.8 |
pg/μg protein |
1.1–1.2 |
pmol/cm3 tissue |
| 60,000–68,000 |
#/EC |
|||
|
|
||||
| VEGFR2 tissue concentration |
||||
| Human vastus lateralis, rest |
0.33–0.5 |
pg/μg protein |
0.24–0.34 |
pmol/cm3 tissue |
| 13,000–19,000 |
#/EC |
|||
|
|
||||
| NRP1 tissue concentration |
||||
| 0.018–1.8 |
pmol/cm3 tissue |
|||
| 1,000–100,000 |
#/EC |
|||
|
|
||||
| ECM binding site density |
||||
| ECM |
0.75 |
μM |
46 |
pmol/cm3 tissue |
| Vessel BM |
13 |
μM |
1 |
pmol/cm3 tissue |
| Myocyte BM |
13 |
μM |
4 |
pmol/cm3 tissue |
|
The conversion of receptor densities to tissue concentrations is based on the relationship mentioned in table 2, and the surface area of an endothelial cell, 1000 μm2. VEGF concentration is normalized based on the entire interstitial space, since it diffuses throughout: 6.2 107 (pmol/cm3 tissue)/M (here, M = moles/liter interstitial space). VEGF binding sites in the ECM and BMs are based on those volumes: 6.2 107 (pmol/cm3 tissue)/M(ECM), 5.7 104 (pmol/cm3 tissue)/M(EBM), 3.1 105 (pmol/cm3 tissue)/M (MBM). For example, M(EBM) = moles/liter endothelial basement membrane. Conversions from pg/mg protein are based on 155 mg protein/g of tissue and 45 kDa VEGF, 210 kDa VEGFR1, 240 kDa VEGFR2 [6]. | ||||
Stefanini et al. BMC Systems Biology 2008 2:77 doi:10.1186/1752-0509-2-77 |
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