|
Kinetic parameters of VEGF in the healthy tissue (human vastus lateralis muscle) |
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| Measured parameters |
Tissue parameters |
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| Value |
Unit |
Value |
Unit |
|
|
|
||||
| VEGF binding to VEGFR1 |
||||
| kon |
3 107 |
M-1 s-1 |
4.8 10-1 |
(pmol/cm3 tissue)-1 s-1 |
| koff |
10-3 |
s-1 |
||
| Kd |
33 |
pM |
2.0 10-3 |
pmol/cm3 tissue |
|
|
||||
| VEGF binding to VEGFR2 |
||||
| kon |
107 |
M-1 s-1 |
1.6 10-1 |
(pmol/cm3 tissue)-1 s-1 |
| koff |
10-3 |
s-1 |
||
| Kd |
100 |
pM |
6.4 10-3 |
pmol/cm3 tissue |
|
|
||||
| VEGF165 binding to NRP1 |
||||
| kon |
3.2 106 |
M-1 s-1 |
5.1 10-2 |
(pmol/cm3 tissue)-1 s-1 |
| koff |
10-3 |
s-1 |
||
| Kd |
312 |
pM |
2.0 10-2 |
pmol/cm3 tissue |
|
|
||||
| VEGF165 binding to GAGs |
||||
| kon |
4.2 105 |
M-1 s-1 |
6.7 10-3 |
(pmol/cm3 tissue)-1 s-1 |
| koff |
10-2 |
s-1 |
||
| Kd |
24 |
nM |
1.5 |
pmol/cm3 tissue |
|
|
||||
| Coupling of NRP1 & VEGFR2 |
||||
| kcV165R2, N1 |
3.1 1013 |
(mol/cm2)-1 s-1 |
2.8 10-1 |
(pmol/cm3 tissue)-1 s-1 |
| koffV165R2,N1 |
10-3 |
s-1 |
||
| kcV165N1,R2 |
1014 |
(mol/cm2)-1 s-1 |
9.2 10-1 |
(pmol/cm3 tissue)-1 s-1 |
| koffV165N1,R2 |
10-3 |
s-1 |
||
|
|
||||
| VEGFR1 coupling to NRP1 |
||||
| kcR1,N1 |
1014 |
(mol/cm2)-1 s-1 |
9.2 10-1 |
(pmol/cm3 tissue)-1 s-1 |
| kdissocR1,N |
10-2 |
s-1 |
||
|
|
||||
| VEGFR internalization |
||||
| kint,R |
2.8 10-4 |
s-1 |
||
| kint,C |
2.8 10-4 |
s-1 |
||
|
The derivation of these parameters is found in [6]. In this table, 6.24 107 (pmol/cm3 tissue)/M and 1.09 1014 (pmol/cm3 tissue)/(mol/cm2 EC). Here, M = moles/liter available interstitial fluid volume. | ||||
Stefanini et al. BMC Systems Biology 2008 2:77 doi:10.1186/1752-0509-2-77 |
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