pH dependence of Ca2+ binding to CaM. Ca2+ binding to CaM under different pH conditions measured by  can be explained in terms of altered dissociation and cooperative dissociation constants. The increase of pH raises affinity to Ca2+ and eliminates cooperativity between Ca2+ binding sites on CaM.
Valeyev et al. BMC Systems Biology 2008 2:48 doi:10.1186/1752-0509-2-48