Figure 5.

Step wise procedure to design genetic decoder. In the first step, circuit designs to produce YFP in absence of glucose and presence of L-arabinose are identified. Next, in step 2, additional elements are appended to the design from the previous step to produce YFP under (-/+) condition and RFP under (-/-) condition. Similarly at step 3 BFP response in introduced. Finally, additional elements are appended to the best designed identified by OptCircuit at step 3 to accomplish the response shown in step 4.