Background
There has been considerable progress and advancement in computational cell biology and this has given researchers powerful tools to simulate metabolic, genetic and signal transduction networks. However there has been little development in designing computational tools to incorporate biologically realistic simulations of systems comprising many interacting biological cells. The necessity to model large ensembles of interacting cells commonly arises in the studies of disease pathology, cancer progression, embryonic development, wound healing and many other important biological processes, and in our case, bacterial quorum sensing. Bacterial quorum sensing has become a very popular area of study in bacteriology. It is a phenomenon in which individual bacteria regulate gene expression by detecting changes in their population density. This is achieved by secretion and detection of auto inducer molecules, whose local concentration increase gives rise to altered gene expression. The ability of bacteria to congregate and reach the threshold density leads to this behavior. This ability might be because of bulk cell growth, or alternatively an efficient method for bacteria to achieve this is by chemotactic motion to form a congregate.
Methodology
We have derived a single cell level probabilistic model based on the cellular dynamics study by Frymer et al. [1] that involves simulation of individual bacterial trajectories. We include a time delay of 0.1 second to account for the time that the bacteria take to tumble. The crucial element in our model design is the tumbling probability (p_{t}) term that causes the bias of the normal randomwalk motion of bacteria in the presence of a chemoattractant.
For a given bacteria with a basal tumbling probability p_{t0}, with a chemotactic coefficient χ_{0}, moving with a velocity v, this term is dependent on mainly the attractant concentration 'a' and the direction vector 'ŝ'.
Results
We first simulated our bacterial population in the absence of any chemoattractant. We expect a random walk behavior. We analyzed the probability density function of the bacterial population and it approached a bivariate normal distribution expected of a random walk at higher time values (100 seconds). We plotted the mean square displacement versus time for various basal tumbling probability (p_{t0}) values. We observed faster convergence to diffusive behavior for lower tumbling probabilities. The convergence times were measured by fitting the radial bacterial displacements (from origin) to a Rayleigh distribution using a ChiSquared Test and thereby extracting the exact times of convergence. This is done for various velocities and basal probabilities. The result is shown in Figure 1. We find that there is an inverse relation between convergence times and tumbling probabilities with a scaling factor of 8.896. We can conclude that the bacteria tumble 9 times before convergence. We also added a simple constant gradient 'ε' is added along the xaxis. The bacterial population moves as a whole with a velocity of V_{c}under the gradient ε. Using Berg & Brown equations [2], we have Vc = mvtanh(ε) The simulation results point at the chemotactic velocity approaching 0.9 of the bacterial velocity. The diffusion along the x and y axes are different in the presence of this gradient. The relation of coefficient in xaxis (μ) to that in y(μ_{0}) is given by μ = mμ_{0}cosh(ε).
Figure 1. Plot of time taken to converge to diffusive behavior versus basal tumbling probability (p_{t0}). Error bars indicate variations for different velocities considered. Overlaid plot of y = constant/p_{t0}.
Under certain conditions, bacteria interact with each other by producing and sensing their own chemotactic signals. These signals include Lasparate, Lserine etc. There have been experiments demonstrating this behavior and formation of a quorum by this phenomenon [3]. We aim to model this using our basic modeling framework. The distinction in this phenomenon is that the chemoattractant gradient field is self generated by bacteria & therefore we need to account for the diffusivity of the attractant along with the bacterial diffusivity. The two basic equations for diffusion of bacterial (b) and diffusion of attractant (a) are thus given by:
There will thus be a transient gradient condition included in our model from the above equation.
Conclusion
The current model describes bacterial motion in the presence of chemoattractant gradient. The population parameters compare very well with models from literature and we plan to test against other existing descriptions. We are incorporating this model into a large scale model that describes the complex cooperative phenomenon in bacteria like quorum sensing. The advantage of single cell level model like ours is that it makes it possible for the model to be incorporated into larger systems like a parallel computational platform [4] and define intracellular dynamics within that system using an agent based modeling technique. Further, this technique can be extended to other biological cells and also in cellcell communication in multicellular organisms.
References

Frymer PD, Ford RM, Cummings PT: Cellular Dynamics Simulation of Bacterial Chemotaxis.
Chemical Engineering Science 1993, 48(5):687699. Publisher Full Text

Berg HC, Brown DA: Chemotaxis in Escherichia coli analyzed by threedimensional tracking.
Nature 1972, 239:500504. PubMed Abstract  Publisher Full Text

Park S, Wolanin PM, Yuzbashyan EA, Silberzan P, Stock JB, Austin RH: Motion to form a quorum.
Science 2003, 301:188. PubMed Abstract  Publisher Full Text

Toh DJ, Tang F, Lee T, Sarda D, Krishnan A, Goryachev AB: Parallel computing platform for the agentbased modeling of multicellular biological systems. In Proceedings of the 5th International Conference on PDCAT: 8–10 December 2004; Singapore. Edited by Liew KM, et Al. SpringerVerilag Berlin Heidelberg; 2004:58. PubMed Abstract