Bicoid profile from FlyEx embryo ms18 after background removal. (A) The spatial index j (μm/5) is the index of 5 μm bins. The fluorescence intensity Ij (circles) were fit to an exponential F[Ij] = a exp(-j/λ) (solid line) with two scaling index lines 1.5F[Ij] and 0.5F[Ij] (dashed). The fluorescence intensity were then converted into log scale in panel (B).
Wu et al. BMC Systems Biology 2007 1:52 doi:10.1186/1752-0509-1-52