Table 2

Sensitivity and specificity values for Paralisacalculated using faecal qPCR as a gold standard
Shedding Threshold for Positivity (organisms/g)
≥102 ≥103 ≥104 ≥105 ≥106 ≥107 ≥108
Paralisa Overall Sens. 0.62 0.67 0.78 0.87 0.89 0.96 0.98
Spec. 0.70 0.68 0.63 0.62 0.61 0.60 0.58
PPV 0.70 0.61 0.41 0.35 0.31 0.26 0.20
NPV 0.62 0.74 0.90 0.95 0.97 0.99 1.00
PPD-J EU >50 Sens. 0.46 0.52 0.70 0.82 0.84 0.91 0.94
Spec. 0.85 0.84 0.81 0.81 0.79 0.78 0.75
PPV 0.78 0.71 0.55 0.50 0.44 0.37 0.28
NPV 0.58 0.70 0.89 0.95 0.96 0.98 0.99
PPD-J EU >100 Sens. 0.34 0.40 0.62 0.74 0.78 0.86 0.89
Spec. 0.94 0.93 0.93 0.92 0.91 0.89 0.86
PPV 0.87 0.82 0.73 0.67 0.62 0.53 0.40
NPV 0.55 0.68 0.88 0.94 0.95 0.98 0.99
PPD-J EU >150 Sens. 0.24 0.29 0.48 0.60 0.64 0.75 0.81
Spec. 0.98 0.98 0.98 0.97 0.96 0.96 0.93
PPV 0.93 0.92 0.88 0.83 0.78 0.71 0.56
NPV 0.53 0.65 0.85 0.91 0.93 0.96 0.98

Sensitivity (Sens.), specificity (Spec.), positive and negative predictive values (PPV and NPV, respectively) for ParalisaTM to predict MAP shedding in deer as determined by qPCR. ‘Paralisa Overall’ indicates the final result when Johnin and Protoplasmic Antigen responses are interpreted in parallel; EU, ELISA Units.

O’Brien et al.

O’Brien et al. BMC Veterinary Research 2013 9:72   doi:10.1186/1746-6148-9-72

Open Data