Table 1

Primer sequences used for the amplification of the various resistance genes
Target genes Sequence (5' to 3') Annealing temp Size (bp) Reference
tetA 5'-GTAATTCTGAGCACTGTCGC-3' 57 950 [22]
5'-CTGCCTGGACAACATTGCTT-3'
tetB 5'-CTCAGTATTCCAAGCCTTTG-3' 52 430 [23]
5'-ACTCCCCTGAGCTTGAGGGG-3'
tetC 5'-GGTTGAAGGCTCTCAAGGGC-3' 62 505 [23]
5'-CCTCTTGCGGGATATCGTCC-3'
tetD 5'-CATCCATCCGGAAGTGATAGC-3' 57 435 [24]
5'-GGATATCTCACCGCATCTGC-3'
tetG 5'-GCAGCGAAAGCGTATTTGCG-3' 62 680 [25]
5'-TCCGAAAGCTGTCCAAGCAT-3'
aadA 5'-ATTTGCTGGTTACGGTGACC-3' 56 533 [21]
5'-CTTCAAGTATGACGGGCTGA-3'
strA 5'-CCAATCGCAGATAGAAGGC-3' 55 500 [26]
5'-CTTGGTGATAACGGCAATTC-3'
strB 5'-GGATCGTAGAACATATTGGC-3' 56 500 [26]
5'-ATCGTCAAGGGATTGAAACC-3'
dfrA14 5'-TGAGAACCTTGAAAGTATCATTG-3' 55 483 This study
5'-ACCCTTTTTCCAAATTTGATAG-3'
florR 5'-ATGGCAGGCGATATTCATTA-3' 55 320 This study
5'-AAACGGGTTGTCACGATCAT-3'
sulII 5'-GCGCTCAAGGCAGATGGCATT-3' 70 284 [26]
5'-GCGTTTGATACCGGCACCCGT-3'
sul1/qacEΔ1I 5'-ATCGCAATAGTTGGCGAAGT-3' 58 798 [22]
5'-GCAAGGCGGAAACCCGCGCC-3
sulI 5'-TGAGATCAGACGTATTGCGC-3' 58 420 This study
5'-TTGAAGGTTCGACAGCACGT-3'
qacEΔ1 5'-ATCGCAATAGTTGGCGAAGT-3' 57 226 [22]
5'-CAAGCTTTTGCCCATGAAGC--3'
IntI1 5'-AAGCAGACTTGACCTGAT-3' 55 Variable [22]
5'-GGCATCCAAGCAGCAAGC-3'
IntI2 5'-CACGGATATGCGACAAAAAGGT-3' 60 789 [27]
5'-GTAGCAAACGAGTGACGAAATG-3'
IntI2 variable 5'-GACGGCATGCACGATTTGTA-3' 56 2214 [28]
5'-GATGCCATCGCAAGTACGAG-3'
gyrA 5'-TACCGTCATAGTTATCCACGA-3' 60 312 [29]
5'-GTACTTTACGCCATGAACGT-3'
parC 5'-CTATGCGATGTCAGAGCTGG-3' 59 261 [29]
5'-TAACAGCAGCTCGGCGTATT-3'

Rahmani et al.

Rahmani et al. BMC Veterinary Research 2013 9:66   doi:10.1186/1746-6148-9-66

Open Data