A) Viral reconstitution. Representative fluorescence and phase contrast images (10X) of BoHV-4-A-ΔgBrevertant virus reconstitution following pBAC-BoHV-4-A-ΔgBrevertant electroporation in BEK or BEK/cre cells. pBAC-BoHV-4-A-ΔgBKanaGalK was unable to reconstitute IRVPs after electroporation in BEK or BEK/cre cells. B) Replication kinetics of BoHV-4-A-ΔgBrevertant compared with BoHV-4-A and BoHV-4-A-ΔgBKanaGalK. The data presented are the means ± standard errors of triplicate measurements.
Franceschi et al. BMC Veterinary Research 2013 9:6 doi:10.1186/1746-6148-9-6