Secreted gB neutralization assay. Representative fluorescence and phase contrast images (10X) of MDBK cells untreated A) or treated B) for 1 hour at 37°C with BoHV-4 gB enriched supernatant and infected with one M.O.I. of BoHV-4-EGFPΔTK. Infection was monitored under a fluorescence microscopy at 24 and 48 hours after the infection. This experiment, along with others made under different condition, as indicated into the text, was repeated three times with identical results.
Franceschi et al. BMC Veterinary Research 2013 9:6 doi:10.1186/1746-6148-9-6