BoHV-4 gB chimeric peptide expression. A) Western Immunoblotting of pWPI/gBFc transfected HEK293 cell lysate or supernatant, in presence or absence of a reducing agent, β-mercaptoethanol. The cell extract of pEGFP-C1 transfected HEK 293 was used as a negative control. As a positive control, the supernatant of cells transfected with a plasmid carrying only the murine Fc was used. B) Western Immunoblotting of pWPI/gBFc and pWPI/gBtruncatedFc transfected HEK293 cell lysates with the presence of β-mercaptoethanol. C) Western Immunoblotting of pWPI/gBgD106 and pWPI/gBtruncatedgD106 transfected HEK293 cell lysates and supernatant . The control used was the same of the Figure 2A .
Franceschi et al. BMC Veterinary Research 2013 9:6 doi:10.1186/1746-6148-9-6