Table 4

Results of testing oro-pharyngeal swabs for avian influenza virus by number of swabs pooled
Swab pool 1 DPI 2 DPI 3 DPI 4 DPI 7 DPI
VI rRT-PCR VI rRT-PCR VI rRT-PCR VI rRT-PCR VI rRT-PCR
1 swab 10/10 (100)* 20/20 (100) 8/10 (80) 19/20 (95) 10/10 (100) 20/20 (100) 10/10 (100) 20/20 (100) 1/10 (10) 16/20 (80)
5 swabs 10/10 (100) 28/30 (93.3) 10/10 (100) 29/30 (96.6) 10/10 (100) 30/30 (100) 10/10 (100) 30/30 (100) 3/10 (30) 26/30 (86.6)
11 swabs 19/20 (95) 29/30 (96.6) 19/20 (95) 30/30 (100) 20/20 (100) 30/30 (100) 20/20 (100) 30/30 (100) 3/20 (15) 25/30 (83.3)

Non-flocked swabs were collected in brain heart infusion broth and were tested by virus isolation and real-time RT-PCR. There were no statistical differences by number of swabs pooled. Abbreviations: DPI = days post inoculation, rRT-PCR = real-time RT-PCR, VI = virus isolation.

*. Number positive/total tested (percent positive).

Spackman et al.

Spackman et al. BMC Veterinary Research 2013 9:35   doi:10.1186/1746-6148-9-35

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