Diagram of experiment to evaluate swab pooling. Specific pathogen free white leghorn chickens were inoculated with 106.5 50% egg infectious doses by in the intrachoanal route. Oro-pharyngeal (OP) and cloacal (CL) swabs were collected at 1, 2, 3, 4, 7 and 14 days post inoculation using non-flocked swabs and brain heart infusion broth. Twenty (2 replicates of 10) individual OP or CL swabs were collected at each time point. Thirty OP and 30 CL (2 replicates of 15) of each of the 5 and 11 swab pools were collected at each time point. Pools were comprised of 1 swab from a chicken inoculated with LPAIV and the remaining (4 or 10) swabs were collected from unexposed chickens.
Spackman et al. BMC Veterinary Research 2013 9:35 doi:10.1186/1746-6148-9-35