Diagram of experiment to evaluate swab type and media type. Specific pathogen free white leghorn chickens were inoculated with 106.5 50% egg infectious doses of AIV by in the intrachoanal route. Fifteen oro-pharyngeal and 15 cloacal swabs were collected at 1, 2, 3, 4, 7, 10, 14, 17 and 21 days post inoculation using non-flocked swabs, flocked swabs and foam swabs. Individual swabs for each swab construction type were transported in either brain heart infusion (BHI) broth or phosphate buffered saline (PBS).
Spackman et al. BMC Veterinary Research 2013 9:35 doi:10.1186/1746-6148-9-35