Additional file 1: Figure S1.

Agarose gels showing DNA pattern of positive samples from the first multiplex reaction with 3 IHHNV primers sets (A) and the second reaction with 2 IHHNV primer sets with an actin-derived primer pair (B). Lanes 1 and 8, DNA ladder; 2, positive control (plasmids containing complete IHHNV genome); 3-6, individual shrimp samples; 7, negative control. Figure S2. Agarose gels showing DNA pattern of samples with putative viral inserts from the first multiplex reaction (A) and the second reaction (B). Lanes 1 and 7, DNA ladder; 2, positive control (plasmids containing entire IHHNV genome); 3-5, individual shrimp samples; 6, negative control. Figure S3. Sensitivity analysis of the first multiplex reaction in (A), and the second reaction in (B). Lanes 1 and 10, DNA ladder; 2, positive control; 3-8, the amount of DNA template varying from 200 ng, 20 ng, 2 ng, 200 pg, 20 pg, 2 pg, respectively; 9, negative.

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Molthathong et al. BMC Veterinary Research 2013 9:33   doi:10.1186/1746-6148-9-33