Open Access Research article

Persistence of Penaeus stylirostris densovirus delays mortality caused by white spot syndrome virus infection in black tiger shrimp (Penaeus monodon)

Sudkhate Molthathong12, Sarocha Jitrakorn23, Yutthana Joyjinda2, Chuenchit Boonchird1, Boonsirm Witchayachamnarnkul24, Pattira Pongtippatee5, Timothy Flegel123 and Vanvimon Saksmerprome23*

Author Affiliations

1 Department of Biotechnology, Faculty of Science, Mahidol University, 10400, Bangkok, Thailand

2 Centex Shrimp, Faculty of Science, Mahidol University, 10400, Bangkok, Thailand

3 National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, 12120, Pathum Thani, Thailand

4 Shrimp Genetics Improvement Center, Surat Thani, Thailand

5 Aquatic Animal Biotechnology Research Center, Surat Thani Campus, 84100, Surat Thani, Thailand

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BMC Veterinary Research 2013, 9:33  doi:10.1186/1746-6148-9-33

Published: 15 February 2013

Additional files

Additional file 1: Figure S1:

Agarose gels showing DNA pattern of positive samples from the first multiplex reaction with 3 IHHNV primers sets (A) and the second reaction with 2 IHHNV primer sets with an actin-derived primer pair (B). Lanes 1 and 8, DNA ladder; 2, positive control (plasmids containing complete IHHNV genome); 3-6, individual shrimp samples; 7, negative control. Figure S2. Agarose gels showing DNA pattern of samples with putative viral inserts from the first multiplex reaction (A) and the second reaction (B). Lanes 1 and 7, DNA ladder; 2, positive control (plasmids containing entire IHHNV genome); 3-5, individual shrimp samples; 6, negative control. Figure S3. Sensitivity analysis of the first multiplex reaction in (A), and the second reaction in (B). Lanes 1 and 10, DNA ladder; 2, positive control; 3-8, the amount of DNA template varying from 200 ng, 20 ng, 2 ng, 200 pg, 20 pg, 2 pg, respectively; 9, negative.

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