p53 protein accumulation in untreated osteosarcoma cells. Representative photomicrographs of cells probed with an antibody against p53 protein show strong accumulation in HMPOS cells, moderate staining in Clone-4 and none in the D17 and COS cell lines. The signal that can be observed in D17 and COS cells is the counterstain hematoxylin (see methods).
Marley et al. BMC Veterinary Research 2013 9:15 doi:10.1186/1746-6148-9-15