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Open Access Highly Accessed Research article

Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota

Petra Videnska, Frantisek Sisak, Hana Havlickova, Marcela Faldynova and Ivan Rychlik*

Author Affiliations

Veterinary Research Institute, Brno, Czech Republic

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BMC Veterinary Research 2013, 9:140  doi:10.1186/1746-6148-9-140

Published: 15 July 2013

Abstract

Background

Infection of newly hatched chicks with Salmonella enterica serovar Enteritidis (S. Enteritidis) results in an inflammatory response in the intestinal tract which may influence the composition of gut microbiota. In this study we were therefore interested whether S. Enteritidis induced inflammation results in changes in the cecal microbiota. To reach this aim, we compared the cecal microbiota of non-infected chickens and those infected by S. Enteritidis by pyrosequencing the V3/V4 variable regions of genes coding for 16S rRNA.

Results

Cecal microbiota of chickens up to 19 days of life was dominated by representatives of Enterobacteriaceae, Lachnospiraceae and Ruminococcaceae, followed by Lactobacillaceae. The presence of Lachnospiraceae did not change after S. Enteritidis infection. Enterobacteriaceae increased and Ruminococcaceae decreased after S. Enteritidis infection in two independent experiments although these results were not significant. A significant increase in both experiments was observed only for the representatives of Lactobacillaceae which may correlate with their microaerophilic growth characteristic compared to the obligate anaerobes from the families Lachnospiraceae and Ruminococcaceae.

Conclusions

We conclude that S. Enteritidis infection influences the composition of the cecal microbiota in chickens but these changes are minor in nature and should be understood more as an indirect consequence of infection and inflammation rather than a positively selected evolutionary trait.

Keywords:
Chicken; Microbiome; Intestinal tract; Pyrosequencing; Salmonella