Table 3

Real time RT-PCR analysis of peptidase genes in Miamiensis avidus under the different culture conditions
Name of clone Mean Cta value (SDb) Fold increase in expression (2−ΔΔCt(SD))
Cell-free culture Cell-feeding culture Cell-free culture Cell-feeding culture
MaPro 1 28.48(0.51) 22.67(0.32) 1.05(0.40) 1.01(0.22)
MaPro 2** 25.38(0.24) 21.25(0.12) 1.01(0.16) 0.31(0.03)
MaPro 3 27.43(0.34) 22.11(0.10) 1.02(0.23) 0.71(0.05)
MaPro 4** 34.96(0.28) 21.95(0.24) 1.02(0.20) 147.58(24.89)
MaPro 5 33.19(0.44) 29.71(0.63) 1.03(0.30) 0.21(0.08)
MaPro 6** 23.93(0.36) 20.01(0.19) 1.02(0.27) 0.27(0.04)
MaPro 7* 33.61(0.31) 25.72(0.11) 1.01(0.22) 4.19(0.33)
MaPro 8** 26.94(0.21) 24.11(0.31) 1.01(0.14) 0.13(0.03)
MaPro 9* 29.33(0.58) 26.79(0.58) 1.05(0.42) 0.11(0.04)
MaPro 10 32.67(1.44) 26.44(0.29) 1.29(0.86) 1.34(0.27)
MaPro 11 30.26(1.30) 25.09(0.25) 1.24(0.79) 0.64(0.11)
MaPro 12** 25.57(0.08) 22.90(0.29) 1.00(0.06) 0.11(0.02)
MaPro 13* 34.37(0.34) 31.15(1.30) 1.02(0.22) 0.21(0.16)
MaPro 14** 32.51(0.41) 19.37(0.01) 1.03(0.27) 159.78(1.40)
MaPro 15 N/Ac 31.12(0.76)
MaPro 16 N/A 31.52(0.57)
MaPro 17* 34.68(0.77) 26.87(0.32) 1.10(0.54) 4.04(0.84)
β-tubulin 23.37(0.45) 17.55(0.22)

aCt: Cycle threshold, bSD: standard deviation, cN/A: No detectable Ct value was obtained within 40 cycles, * : Significant differences P<0.05, ** : Significant differences P<0.005.

Seo et al.

Seo et al. BMC Veterinary Research 2013 9:10   doi:10.1186/1746-6148-9-10

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