Table 2

Association between studied variables with disease-free and overall survival times
Variable Animals Recurrence or distant metastases Mean DFS time in months Р* Dead or euthanized dogs Mean OS time in months Р*
N % N % (± SE) N % (± SE)
Total 85 100 26 30.6 5.3 (5.7) 22 25.8 11.9 (7.6)
Animal factors
Weight (kg) NS NS
<10 27 31.8 7 25.9 19.6 (1.6) 6 22.2 21.7 (1.2)
10-23 39 45.9 11 28.2 18.3 (1.5) 10 25.6 20.0 (1.2)
>23 19 22.3 8 42.1 15.8 (2.2) 6 31.6 19.2 (1.8)
Spayed NS NS
No 72 84.7 21 29.2 18.4 (1.1) 17 23.6 20.7 (0.8)
Yes 13 15.3 5 38.5 16.8 (2.7) 5 38.5 19.3 (1.9)
Hormonal therapyb NS NS
No 74 90.2 23 31.1 18.0 (1.1) 19 25.7 20.5 (0.8)
Yes 8 9.8 2 25.0 19.5 (2.9) 2 25.0 20.7 (2.4)
Tumour factors
Ulceration NS NS
No 74 87.1 21 28.4 18.6 (1.0) 17 23.0 21.0 (0.8)
Yes 11 12.9 5 45.5 14.6 (3.2) 5 45.5 16.6 (2.8)
Size 0.027 0.016
< 3 cm 47 55.3 10 21.3 20.3 (1.1) 8 17.0 22.1 (0.8)
≥ 3 cm 38 44.7 16 42.1 15.5 (1.7) 14 36.8 18.3 (1.3)
Necrosis NS NS
No 25 29.4 7 28.0 18.3 (1.9) 7 28.0 20.1 (1.5)
Yes 60 70.6 19 31.2 18.0 (1.2) 15 25.0 20.5 (0.9)
Squamous metaplasia NS NS
No 62 72.9 19 30.1 18.1 (1.2) 17 27.4 20.5 (0.9)
Yes 23 27.1 7 30.4 18.1 (1.9) 5 21.7 20.4 (1.6)
Mode of growth 0.007 0.022
Expansive 24 28.2 2 8.3 22.6 (0.9) 2 8.3 23.3 (0.7)
Invasive 61 71.8 24 39.3 16.4 (1.3) 20 32.8 19.3 (0.9)
Histological type NS 0.023
Complex carcinomas 20 23.5 4 20.0 20.7 (1.6) 2 10.0 23.6 (0.3)
Simple carcinomas 51 60.0 15 29.4 18.5 (1.3) 13 25.5 20.6 (0.9)
Others 14 16.5 7 50.0 13.1 (2.9) 7 50.0 15.4 (2.4)
Mitotic index NS NS
0-9/10hpf 36 42.4 10 27.8 18.6 (1.5) 9 25.0 20.9 (1.2)
10-19/10hpf 25 29.4 6 24.0 18.9 (1.8) 4 16.0 21.2 (1.3)
≥ 20/10hpf 24 28.2 10 41.7 16.7 (1.9) 9 37.5 19.1 (1.4)
Histological grade NS 0.025
I + II 58 68.2 14 24.1 19.3 (1.1) 11 19.0 21.8 (0.8)
III 27 31.8 12 44.4 15.5 (1.9) 11 40.7 17.7 (1.6)
RLN metastasesa 0.017 0.005
No 47 71.2 10 21.3 20.2 (1.1) 8 17.0 22.2 (0.7)
Yes 19 28.8 9 47.4 14.6 (2.4) 9 47.4 17.3 (2.1)
Cancer cells
MIB1 L.I. (%) <0.001 <0.001
< 40 47 55.3 7 14.9 21.1 (1.03) 6 12.8 22.8 (0.7)
≥ 40 38 44.7 19 50.0 14.4 (1.66) 16 42.1 17.5 (1.4)
TIMP-2 (%) NS NS
< 25 32 37.6 7 21.9 19.7 (1.5) 5 15.6 21.6 (1.1)
≥ 25 53 62.4 19 35.8 17.1 (1.3) 17 32.1 19.7 (1.0)
VEGF-A (%) NS NS
< 50 47 55.3 14 29.8 18.4 (1.3) 10 21.3 21.1 (0.9)
≥ 50 38 44.7 12 31.6 17.7 (1.5) 12 31.6 19.7 (1.0)
Stromal cells
uPA (%) 0.016 0.012
< 10 25 29.4 3 12.0 21.9 (1.1) 2 8.0 23.5 (0.4)
≥ 10 60 70.6 23 38.3 16.5 (1.3) 20 33.3 19.1 (1.0)
MMP-9 (%) <0.001 <0.001
< 50 54 63.5 9 16.7 20.9 (0.9) 8 14.8 22.5 (0.7)
≥ 50 31 36.5 17 54.8 13.2 (1.9) 14 45.2 16.7 (1.5)

a Lymph nodes were not submitted in 19 cases.

b The information regarded hormonal therapy was impossible to obtain in three cases.

*Log Rank test.

NS – not significant.

Santos et al.

Santos et al. BMC Veterinary Research 2013 9:1   doi:10.1186/1746-6148-9-1

Open Data