Table 2

List of primers, their sequences, amplification conditions and locus details
Primer name Position on MAP K10 genome, acc. no AE016958 Sequence Target Thermal conditions (x = No. of cycles) Reference
IS900/150 C 39946-39969 5´-CCGCTAATTGAGAGATGCGATTGG-3’ IS900 95 °C-3 min, 45x: 95 °C-30 sec,61 °C-30 sec; 72 °C-20 sec; 72 °C-3 min [32]
IS900/921 40174-40150 5´-AATCAACTCCAGCAGCGCGGCCTCG-3’
MA901/373up Not applicable 5’-CGGATTGCTAACCACGTGGTGTGTG-3’ IS901 95 °C-3 min, 45x: 95 °C-30 sec; 60 °C-30 sec; 72 °C-40 sec; 72 °C-3 min [25]
MA901/956do Not applicable 5’-GTGCTGCGAGTTGCTTGATGAG-3’
IS1311auF (M56) 33911- 33930 5’-GCGTGAGKCTCTGTGGTGAA-3’ IS1311 95 °C-3 min, 45x: 95 °C - 30 sec; 59 °C - 30 sec; 72 °C-40 sec; 72 °C-3 min [11,19] and this study
M119 33323-33340 5’-ATGACGACCGCTTGGGAG-3’
M2/F 3253559-3253583 5’-CCTGCTCGACGAACACCTCAA-3’ MIRU 2 95 °C-3 min, 47x: 97 °C-30 sec; 60 °C-30 sec; 72 °C-60 sec; 72 °C-3 min This study
M2/R 3253860-3253834 5’-CGAAGATCCTGGGACTGGACGAGTTGG-3
M3/F 4441771-4441797 5’-CGGATCGACATTCACCCTGTCCATTCC-3’ MIRU 3 95 °C-3 min, 47x: 97 °C-30 sec; 57 °C-30 sec; 72 °C-60 sec; 72 °C-3 min This study
M3/R 4442081-4442059 5’-CGAAGCCCTCCTTACGGAGCAGG-3’
M32/F 115702-1125726 5’-CAGCGCCACAGGGTTTTTGGTGAAG-3’ VNTR 32 95 °C-3 min, 47x: 97 °C-30 sec; 60 °C-30 sec; 72 °C-60 sec; 72 °C-3 min This study
M32/R 1126006-1125981 5’-GCGGAAATCCAACAGCAAGGACGGAC-3’
SRL1F 1793439-1793420 5’- GGTGTTCGGCAAAGTCGTTGTGCC-3 SSR 1 95 °C-3 min, 47x: 97 °C-30 sec; 61 °C-30 sec; 72 °C-40 sec; 72 °C-3 min This study
SRL1R 1792995-1793014 5’-GCGCGTCAGACTGTGCGGTATGG-3’
SRL2F 2719265-2719287 5’-CGGCTGCACTTGCACGACTCTAGG-3’ SSR 2 95 °C-3 min, 47x: 97 °C-30 sec; 64 °C-60 sec; 72 °C-40 sec; 72 °C-3 min This study
SRL2R 2718879-2718902 5’-GCGTAGCGTTGTGGGCTTGGAGG-3’
SLR8F 1027929-1027950 5’-GCGAGATGTCGACCATCCTGAC-3’ SSR 8 95 °C-3 min, 47x: 97 °C-30 sec; 59 °C-30 sec; 72 °C-20 sec; 72 °C-3 min This study
SLR8R 1028263-1028283 5’-CGCTCGACGATCAGCGTGTTG-3’

Okuni et al.

Okuni et al. BMC Veterinary Research 2012 8:99   doi:10.1186/1746-6148-8-99

Open Data