Figure 2.

Comparison of HEPES (DGHB⁺⁺) and Veronal (DGVB⁺⁺) buffers. Serial 2-fold dilutions of human serum were prepared in either buffer, starting at 1/20 (5%). Dilutions (100 μl) were mixed with 100 μl of 10⁸/ml sheep EA cells, in the same buffer, and incubated for 1hour, at 37 °C. Percentage lysis was calculated as in methods.