Comparison of HEPES (DGHB++) and Veronal (DGVB++) buffers. Serial 2-fold dilutions of human serum were prepared in either buffer, starting at 1/20 (5%). Dilutions (100 μl) were mixed with 100 μl of 108/ml sheep EA cells, in the same buffer, and incubated for 1hour, at 37 °C. Percentage lysis was calculated as in methods.
Moreno-Indias et al. BMC Veterinary Research 2012 8:91 doi:10.1186/1746-6148-8-91