Table 1

List of identified sheep serum proteins
SPOT N° (a) NAME ACCESSION CODE(b) Theoretical Mr/pI Experimental Mr/pI (e) # peptides MS # total peptides MS/MS Mascot SCORE(c) Orthologue from(d)
1 α 1 Antitripsin A1AT_SHEEP 45.984/5.83 63.0/4.47 17 2 279 m
2 α 1 Antitripsin A1AT_SHEEP 45.984/5.83 62.8/4.55 14 10 311 e
3 α 1 Antitripsin A1AT_SHEEP 45.984/5.83 63.7/4.40 16 9 249 e
4 α 1 Antitripsin A1AT_SHEEP 45.984/5.83 50.5/4.43 7 2 45 m
5 α 1 Antitripsin A1AT_SHEEP 45.984/5.83 50.4/4.52 9 3 99 m
6 α 1B Glycoprotein A1BG_BOVIN 53.553/5.30 74.1/4.39 16 5 176 m Bovine
7 α 1B Glycoprotein A1BG_BOVIN 53.553/5.30 73.3/4.48 9 4 146 m Bovine
8 α 1 acid Glycoprotein A1AG_BOVIN 23.182/5.62 46.1/3.35 6 2 82 m Bovine
9 Amine Oxidase AOCX_BOVIN 84.756/5.57 90.1/5.10 19 5 161 m Bovine
10 Apolipoprotein A1 O02762_SHEEP 66.173/4.61 25.2/5.24 18 8 256 m
11 Apolipoprotein A1 O02762_SHEEP 66.173/4.61 25.7/5.01 11 3 137 m
12 Apolipoprotein A1 O02762_SHEEP 66.173/4.61 26.0/4.80 31 16 650 m
13 Apolipoprotein A1 O02762_SHEEP 66.173/4.61 26.2/4.63 13 2 235 m
14 Apolipoprotein A1 O02762_SHEEP 66.173/4.61 26.0/5.51 15 8 273 e
15 Ceruloplasmin CERU_SHEEP 119.126/5.50 137.2/5.06 16 5 267 m
16 Ceruloplasmin CERU_SHEEP 119.126/5.50 137.8/5.15 15 7 279 m
17 Ceruloplasmin CERU_SHEEP 119.126/5.50 137.1/5.01 14 6 218 m
18 Protein kinase KAPCA_SHEEP 40.589/8.71 32.5/3.66 11 3 95 m
19 Complement component 3 O97941_BOVIN 17.044/5.25 67/9.38 5 2 81 m Bovine
20 Corticotropin releasing factor CRFR1_SHEEP 47.558/9.63 93.7/5.46 7 2 62 m
21 Fetuin Precursor FETUA_SHEEP 38.680/5.17 69.7/4.02 7 4 157 m
22 Fetuin Precursor FETUA_SHEEP 38.680/5.17 56.2/4.04 8 3 116 m
23 α 1B Glycoprotein A1BG_BOVIN 53.553/5.30 75.6/4.16 14 6 218 m Bovine
24 α 1B Glycoprotein A1BG_BOVIN 53.553/5.30 74.8/4.23 12 6 239 m Bovine
25 α 1B Glycoprotein A1BG_BOVIN 53.553/5.30 74.5/4.26 21 13 366 m Bovine
26 α 1B Glycoprotein A1BG_BOVIN 53.553/5.30 73.9/4.35 11 2 88 m Bovine
27 Fibrinogen α chain FIBA_BOVIN 67.012/6.73 56.0/6.31 6 2 81 m Bovine
28 Fibrinogen β chain FIBB_BOVIN 53.339/8.45 56.6/6.00 14 7 175 e Bovine
29 Fibrinogen β chain FIBB_BOVIN 53.339/8.45 55.0/6.54 16 6 200 m Bovine
30 Fibrinogen β chain FIBB_BOVIN 53.339/8.45 55.0/6.70 11 4 132 m Bovine
31 Fibrinogen α chain FIBA_BOVIN 67.012/6.73 74.4/6.96 5 2 90 m Bovine
32 Fibrinogen α chain FIBA_BOVIN 67.012/6.73 74.7/6.86 4 2 78 m Bovine
33 Fibrinogen α chain FIBA_BOVIN 67.012/6.73 74.0/6.72 11 3 73 m Bovine
34 Fibrinogen α chain FIBA_BOVIN 67.012/6.73 74.4/6.78 5 2 61 m Bovine
35 Fibrinogen α chain FIBA_BOVIN 67.012/6.73 65.3/6.94 9 2 87 m Bovine
36 Fibrinogen β chain FIBB_BOVIN 53.339/8.45 65.3/6.81 17 4 130 m Bovine
37 Gelsolin F2YQ13_SHEEP 80.719/5.59 94.4/5.87 13 4 156 m
38 Haptoglobin HPT_BOVIN 44.859/7.83 36.7/6.38 9 4 185 m Bovine
39 Haptoglobin HPT_BOVIN 44.859/7.83 36.6/6.56 18 5 222 m Bovine
40 Haptoglobin HPT_BOVIN 44.859/7.83 36.6/6.53 17 4 182 m Bovine
41 Haptoglobin HPT_BOVIN 44.859/7.83 36.6/6.66 7 2 76 m Bovine
42 Hemoglobin β HBB_SHEEP 16.073/6.75 12.7/7.82 23 8 516 m
43 Hemoglobin β HBB_SHEEP 16.073/6.75 12.6/8.24 21 7 428 m
44 α globin chain Q28745_SHEEP 15.178/7.94 12.2/8.70 17 11 306 m
45 α globin chain Q28745_SHEEP 15.178/7.94 12.2/9.72 18 10 491 m
46 Ig λ -1 chain CAA494511 51.564/5.65 41.0/8.69 4 3 141 e
47 Ig λ -1 chain CAA494511 51.564/5.65 41.0/8.82 7 5 158 e
48 Ig λ -1 chain CAA494511 51.564/5.65 41.0/8.97 7 3 137 e
49 Ig λ -1 chain CAA494511 51.564/5.65 51.4/8.65 8 6 150 e
50 Ig mu chain AAA513791 53.545/5.10 80.9/5.31 17 12 319 m
51 Ig mu chain AAA513791 53.545/5.10 80.9/5.45 16 12 314 m
52 Apolipoprotein A1 AAI02942 30.276/5.71 26.9/5.68 24 7 399 m Bovine
IgG α λ-chain AAU45093 11.328/8.48 26.9/5.68 19 10 337 m
53 IgG α λ-chain AAU45093 11.328/8.48 26.6/9.11 14 6 202 m
54 IgG α λ-chain AAU45093 11.328/8.48 26.7/8.27 9 3 113 m
55 IgG α λ-chain AAU45093 11.328/8.48 27.6/6.39 14 5 286 m
56 IgG α λ-chain AAU45093 11.328/8.48 26.4/9.43 11 4 146 m
57 IgG α λ-chain AAU45093 11.328/8.48 51.2/8.78 7 2 98 m
58 Ig λ -1 chain CAA494511 51.564/5.65 51.1/8.69 5 2 68 m
59 IgG α λ-chain AAU45093 11.328/8.48 26.1/9.95 13 2 118 m
60 IgG α λ-chain AAU45093 11.328/8.48 27.1/5.13 7 3 115 m
61 Ig mu chain AAA513791 53.545/5.10 52.4/6.85 8 2 102 m
62 α Tripsin Inhibitor Chain H4 ITIH4_BOVIN 101.512/6.22 119.4/5.52 7 2 82 m Bovine
63 Muscle Endopin 1b SPA33_BOVIN 46.326/6.05 126.3/4.18 6 2 88 m Bovine
64 Muscle Endopin 1b SPA33_BOVIN 46.326/6.05 126.4/4.28 16 4 142 m Bovine
65 Retynol-Binding protein RET3_SHEEP 27.992/3.49 22.2/5.59 14 6 287 m
66 Serum albumin ALBU_SHEEP 69.188/5.80 44.3/5.22 13 8 236 m
67 Serum albumin ALBU_SHEEP 69.188/5.80 42.0/5.23 12 6 193 e
68 Serum albumin ALBU_SHEEP 69.188/5.80 39.8/5.53 19 10 359 m
69 Serum albumin ALBU_SHEEP 69.188/5.80 34.7/5.52 17 7 279 m
70 Serum albumin ALBU_SHEEP 69.188/5.80 46.6/5.23 9 4 138 m
71 Serum albumin ALBU_SHEEP 69.188/5.80 34.8/5.95 14 8 258 m
72 Serum albumin ALBU_SHEEP 69.188/5.80 35.6/5.77 15 6 212 m
73 Serum albumin ALBU_SHEEP 69.188/5.80 16.5/4.68 12 5 167 e
74 Serum albumin ALBU_SHEEP 69.188/5.80 16.1/5.05 16 7 222 m
75 Serum albumin ALBU_SHEEP 69.188/5.80 21.8/4.53 15 9 326 m
76 Serum albumin ALBU_SHEEP 69.188/5.80 22.1/4.47 15 10 332 m
77 Serum albumin ALBU_SHEEP 69.188/5.80 23.9/4.41 16 9 335 m
78 Serum albumin ALBU_SHEEP 69.188/5.80 11.2/4.36 11 5 176 e
79 Serum albumin ALBU_SHEEP 69.188/5.80 11.3/4.06 8 2 93 e
80 Serum albumin ALBU_SHEEP 69.188/5.80 9.6/3.93 7 4 119 m
81 Serum albumin ALBU_SHEEP 69.188/5.80 116.4/5.69 7 2 71 m
82 Serum albumin ALBU_SHEEP 69.188/5.80 116.3/5.74 10 2 115 e
83 Serum albumin ALBU_SHEEP 69.188/5.80 93.5/5.53 21 11 353 m
84 Serum albumin ALBU_SHEEP 69.188/5.80 92.8/6.08 19 8 275 m
85 Serum albumin ALBU_SHEEP 69.188/5.80 31.3/6.29 18 6 248 m
86 Serum albumin ALBU_SHEEP 69.188/5.80 31.3/6.55 13 5 173 m
87 Serum albumin ALBU_SHEEP 69.188/5.80 30.8/6.83 14 7 273 m
88 Serum albumin ALBU_SHEEP 69.188/5.80 27.4/6.70 15 6 237 m
89 Serum albumin ALBU_SHEEP 69.188/5.80 25.5/6.94 16 6 254 m
90 Serum albumin ALBU_SHEEP 69.188/5.80 26.4/7.03 15 8 270 m
91 Serum albumin ALBU_SHEEP 69.188/5.80 26.4/7.25 14 5 195 m
92 Serum albumin ALBU_SHEEP 69.188/5.80 25.7/7.16 16 5 219 m
93 Serum albumin ALBU_SHEEP 69.188/5.80 36.5/6.26 13 7 265 m
94 Serum albumin ALBU_SHEEP 69.188/5.80 74.3/3.05 15 7 261 m
95 Serum albumin ALBU_SHEEP 69.188/5.80 27.2/5.40 5 2 75 m
96 Serum albumin ALBU_SHEEP 69.188/5.80 24.7/6.24 8 4 161 m
97 Serum albumin ALBU_SHEEP 69.188/5.80 25.5/6.38 5 2 88 e
98 Serum albumin ALBU_SHEEP 69.188/5.80 24.6/6.69 17 10 369 m
99 Serum albumin ALBU_SHEEP 69.188/5.80 93.5/5.68 14 7 222 m
100 Serum albumin ALBU_SHEEP 69.188/5.80 43.0/4.76 6 2 87 m
101 Serum albumin ALBU_SHEEP 69.188/5.80 49.3/6.46 23 13 456 e
102 Serum albumin ALBU_SHEEP 69.188/5.80 49.4/6.30 21 12 401 m
103 Serum albumin ALBU_SHEEP 69.188/5.80 63.704 15 8 276 m
104 Serum albumin ALBU_SHEEP 69.188/5.80 63.9/6.08 22 13 461 m
105 Serum albumin ALBU_SHEEP 69.188/5.80 150.2/5.64 21 10 362 m
106 Transferrin O18780_SHEEP 66.454/8.29 77.5/6.44 12 4 125 m
107 Transferrin O18780_SHEEP 66.454/8.29 77.3/6.33 13 4 145 m
108 Transferrin O18780_SHEEP 66.454/8.29 76.9/6.54 8 3 142 e
109 Transferrin O18780_SHEEP 66.454/8.29 82.1/6.66 18 8 279 m
110 Transferrin O18780_SHEEP 66.454/8.29 82.8/6.22 6 2 60 m
111 Transferrin O18780_SHEEP 66.454/8.29 82.1/6.32 6 3 117 m
112 Transthyretin TTHY_SHEEP 15.770/5.59 13.0/5.87 15 9 375 e
113 Transthyretin TTHY_SHEEP 15.770/5.59 13.1/5.42 18 14 479 e
114 Apolipoprotein AII APOA2_BOVIN 11.201/7.80 10.0/4.03 5 2 77 m Bovine
115 VitD Binding Protein VTDB_BOVIN 53.342/5.36 64.7/4.48 5 2 104 m Bovine
116 VitD Binding Protein VTDB_BOVIN 53.342/5.36 64.7/4.56 5 2 46 m Bovine
117 VitD Binding Protein VTDB_BOVIN 53.342/5.36 63.2/4.86 4 2 40 m Bovine
118 VitD Binding Protein VTDB_BOVIN 53.342/5.36 62.9/4.92 5 2 59 e Bovine
119 Zn A2 Glycoprotein ZA2G_BOVIN 33.851/5.11 50.2/3.66 13 7 271 m Bovine
120 Factor XIIa Inhibitor F12AI_BOVIN 51.723/6.19 88.4/4.06 6 2 82 e Bovine
121 Factor XIIa Inhibitor F12AI_BOVIN 51.723/6.19 88.1/4.12 6 3 119 m Bovine
122 Factor XIIa Inhibitor F12AI_BOVIN 51.723/6.19 87.8/4.18 9 3 148 m Bovine
123 Ig mu chain AAA513791 53.545/5.10 82.8/4.88 13 3 120 m
124 Ig mu chain AAA513791 53.545/5.10 81.8/5.01 14 5 152 m
125 Ig λ -6a light chain AAB94911 11.718/4.65 24.2/4.87 11 4 135 m
126 Ig G heavy chain AAB37381 35.848/6.09 53.8/6.31 7 2 68 m Bovine
127 Paraoxonase 1 Q2KIW1_BOVIN 39.841/5.24 46.8/3.89 9 4 137 m Bovine
128 Paraoxonase 1 Q2KIW1_BOVIN 39.841/5.24 46.6/4.01 8 3 122 e Bovine
129 Muscle Endopin 1b SPA33_BOVIN 46.326/6.05 126.6/4.38 10 6 192 m Bovine
130 Muscle Endopin 1b SPA33_BOVIN 46.326/6.05 126.4/4.58 11 7 204 m Bovine
131 Fetuin FETUA_SHEEP 38.680/5.17 68.1/4.06 8 2 83 m
132 Fetuin FETUA_SHEEP 38.680/5.17 67.7/4.12 10 4 172 m
133 Fetuin FETUA_SHEEP 38.680/5.17 66.2/4.01 12 7 209 m
134 Fetuin FETUA_SHEEP 38.680/5.17 56.2/4.42 11 5 163 m
135 Gelsolin F2YQ13_SHEEP 80.719/5.59 94.4/5.87 10 6 214 e
136 Gelsolin F2YQ13_SHEEP 80.719/5.59 92.8/6.08 13 8 245 e
137 Corticotropin 550074A 45.421/6.51 93.6/5.30 17 11 316 m
138 Corticotropin 550074A 45.421/6.51 93.9/5.38 10 4 141 m

a) Number refer to protein spots indicated in Figures.

b) Accession number from SWISS-PROT or NCBI entry is given.

c) MOWSE score required for significant homology (P < 0.05); "m" indicates MALDI-TOF/TOF-MS; "e" indicates CE-ESI-MS.

d) Sheep protein or homologue to.

e) Values for Mr and pI experimentally determined for each spot according to marker proteins.

Chiaradia et al.

Chiaradia et al. BMC Veterinary Research 2012 8:66   doi:10.1186/1746-6148-8-66

Open Data