Strategy used to construct FMDV O/HN/CHA/93 full-length cDNA clone and genetically modified clone. The location of the restriction enzyme cleavage sites used to assemble the subcloned PCR fragments (Z1, Z2, Z3 and Z4) are shown (numbered relative to nucleotide position in the virus genome). Thick lines and an open box represent the untranslated regions and the open-reading frame for the viral polyprotein, respectively. The thin line represents the vector sequence. FMDV cDNA is under the control of the T7 promoter.
Li et al. BMC Veterinary Research 2012 8:57 doi:10.1186/1746-6148-8-57