The growth curves of EPCs under different culture conditions. The growth curves of EPCs appeared sigmoidal under the “Media A & Gelatin” culture conditions. Other curves did not appear sigmoidal. The population doubling times (PDT) were approximately 31.9 h (Media A & Gelatin), 40.5 h (Media B & Gelatin), 43.6 h (Media A & Fibronectin) and 52.7 h (Media B & Fibronectin). “Media A & Gelatin” was determined to provide the optimal conditions for the proliferation of EPCs in vitro.
Bai et al. BMC Veterinary Research 2012 8:54 doi:10.1186/1746-6148-8-54