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Open Access Highly Accessed Methodology article

Evaluation of combined high-efficiency DNA extraction and real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in subclinically infected dairy cattle: comparison with faecal culture, milk real-time PCR and milk ELISA

Katarina Logar1*, Rok Kopinč2, Petra Bandelj1, Jože Starič3, Aleš Lapanje2 and Matjaž Ocepek1

Author Affiliations

1 Veterinary Faculty, Institute of Microbiology and Parasitology, Gerbičeva 60, Ljubljana, 1115, Slovenia

2 Institute of Physical Biology, Toplarniška 19, Ljubljana, 1000, Slovenia

3 Veterinary Faculty, Clinic for Ruminants, Gerbičeva 60, Ljubljana, 1115, Slovenia

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BMC Veterinary Research 2012, 8:49  doi:10.1186/1746-6148-8-49

Published: 2 May 2012

Additional files

Additional file 1:

Bacterial strains used to test the specificity of the IS900 quantitative real-time PCR. The reference and clinical Map and non-Map strains used to test the specificity of the IS900 quantitative real-time PCR are listed in the file.

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