Additional file 1.
Brain tissue from 20 Tg338 passage 2 mice (five from each sheep- or goat-inoculated P1 donor) were selected for western blot assay and glycoform analysis. Three hundred μg wet brain weight equivalent volumes of each of the mouse brain were digested with proteinase K and loaded into SDS-PAGE gels followed by western blot assay using mAb F99/97.6.1 and chemiluminescent imaging. Films (representative film shown in Additional File 1) were used to obtain relative densities of un-, mono- and di-glycosylated forms of PrPSc bands for glycoform analysis.
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O’Rourke et al. BMC Veterinary Research 2012 8:42 doi:10.1186/1746-6148-8-42