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Transmissibility of caprine scrapie in ovine transgenic mice

Katherine I O’Rourke1*, David A Schneider1, Terry R Spraker2, Rohana P Dassanayake3, Margaret A Highland3, Dongyue Zhuang1 and Thomas C Truscott1

Author Affiliations

1 United States Department of Agriculture, Agricultural Research Service, Pullman, WA, 99164, USA

2 Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, 80526, USA

3 Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164, USA

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BMC Veterinary Research 2012, 8:42  doi:10.1186/1746-6148-8-42

Published: 2 April 2012

Additional files

Additional file 1:

Brain tissue from 20 Tg338 passage 2 mice (five from each sheep- or goat-inoculated P1 donor) were selected for western blot assay and glycoform analysis. Three hundred μg wet brain weight equivalent volumes of each of the mouse brain were digested with proteinase K and loaded into SDS-PAGE gels followed by western blot assay using mAb F99/97.6.1 and chemiluminescent imaging. Films (representative film shown in Additional File 1) were used to obtain relative densities of un-, mono- and di-glycosylated forms of PrPSc bands for glycoform analysis.

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Additional file 2:

Glycoform patterns of PrPSc from 3 goats and a reference sheep with naturally occurring scrapie and from Tg338 mice from each inoculum group.

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Open Data