Table 1

Non specific binding responses of T.gondii and T. spiralis negative serum sets and conjugate alone

Serum

Bead identification

n

Min. response (MFI)

Max. response (MFI)

Mean response (MFI)

SE


Buffer

REF

98

300

600

500

5


TOX

98

3,900

4,700

4,300

19


TRI

98

3,200

3,900

3,500

17


(T. gondii or T. spiralis) negative field sera

REF

947

400

491,500

53,700

2,200


TOX

932

4,200

269,200

47,900

1,100


TRI

945

3,400

505,500

75,300

2,300


Experimental infection negative control sera

REF

36

1,100

1,900

4,100

600


TOX

36

4,900

45,500

21,500

1,900


TRI

36

4,200

34,800

10,400

1,000


Minimum, maximum and mean response signals of reference (REF), T. gondii (TOX) and T. spiralis (TRI) coupled beads after incubation of buffer, negative field sera (without positive responders in T. gondii ELISA) and sera of the negative control animals of the experimental infection. n, number of tested sera; SE, standard error

Bokken et al. BMC Veterinary Research 2012 8:36   doi:10.1186/1746-6148-8-36

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