Table 4

Morphology score criteria applied in the initial media testing

score

Proliferation (P0)

Proliferation (P1)

Vacuole formation

Phenotype


3

Continuous margin of cells around explants

> 90% confluence

no vacuoles

-

2

Discontinuous margin of cells around explants

70%-90% confluence

< 10% cells vacuolated

epitheloid phenotype

1

single cells and small clusters of cells around explants

< 70% confluence

> 10% cells vacuolated

-

0

not assessable, all or many cells dead

not assessable, all or many cells dead

not assessable, all or many cells dead

fibroblastoid phenotype


P0 primary cells, P1 cells in passage one

Miessen et al. BMC Veterinary Research 2012 8:31   doi:10.1186/1746-6148-8-31

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