Table 2

Antibodies and protocol details for immunohistochemistry (IHC) and immunocytochemistry (ICC) procedures

name

manufacturer

dilution

blocking solution

IHC

Beta Catenin, ab6302

Abcam plc, Cambridge, Uk

1:160.000 in PBS

no block

Anti human cytokeratin, clones AE1/AE3

DakoCytomation, Glostrup, Denmark

2.4 μg/mL in PBS

Casein, Candor Bioscience, Münster, Germany

ICC

Beta Catenin, ab6302

Abcam plc, Cambridge, Uk

1:2.000 in PBS

no block

Anti human cytokeratin, clones AE1/AE3

DakoCytomation, Glostrup, Denmark

1 μg/mL in PBS

no block

Miessen et al. BMC Veterinary Research 2012 8:31   doi:10.1186/1746-6148-8-31