Immunofluorescence analysis of primary cultures for mammary malignant epithelial cell markers. Cells were stained with relevant markers (CK14, CK18, EMA, ER-α) for epithelial mammary cells frequently over-expressed proteins in malignant mammary tumors (HER2/neu, EGFR): immunopositivity demonstrates the presence in feline primary carcinoma cultures the presence of malignant epithelial mammary cells. Representative confocal microscopy images are reported. Bar 25 μM, original magnification 60×.
Ferrari et al. BMC Veterinary Research 2012 8:27 doi:10.1186/1746-6148-8-27