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Open Access Research article

The effect of agility exercise on eicosanoid excretion, oxidant status, and plasma lactate in dogs

Wendy I Baltzer1*, Anna M Firshman2, Bernadette Stang1, Jennifer J Warnock1, Elena Gorman1 and Erica C McKenzie1

Author affiliations

1 Departments of Clinical Sciences (Baltzer, Firshman, Stang, Warnock, McKenzie) and Biomedical Sciences (Gorman), College of Veterinary Medicine, Oregon State University, Corvallis, OR, 97331, USA

2 Present address: 225 Veterinary Medical Center, 1365 Gortner Ave, St. Paul, MN, 55108, USA

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Citation and License

BMC Veterinary Research 2012, 8:249  doi:10.1186/1746-6148-8-249

Published: 28 December 2012

Abstract

Background

The objective was to determine the effects of agility exercise on dogs of different skill levels with respect to urinary eicosanoids, urinary 15F2t-isoprostane (lipid peroxidation marker) and hematological/biochemical changes in plasma. Fifteen adult dogs had blood and urine samples obtained prior to, immediately and 4-hours following an agility exercise.

Results

Hematocrit, red blood cells (RBC), albumin, and hemoglobin increased following exercise, with greatest increases correlating to increased skill group (novice, intermediate, masters); at 4-hours post-exercise, hematocrit, RBC, and hemoglobin were decreased. Phosphorus increased following exercise with the greatest increase in novice and intermediates. Plasma lactate increased 3.6-fold in masters, 3.2-fold in intermediates, and 1.2-fold in novice dogs. Urine thromboxane B2 (TXB2) more than tripled 4-hours post-exercise while 6-keto prostaglandin F (PGF1α, prostacyclin metabolite), prostaglandin E2 metabolites (13,14-dihydro-15-keto-prostaglandin A2 and 13,14-dihydro-15-keto-prostaglandin E2), and 13,14-dihydro-15-keto prostaglandin F were unaffected as determined by a competitive enzyme immunoassay and standardized by division with urine creatinine. Urine 15F2t-isoprostane increased insignificantly.

Conclusions

Alterations in the plasma post-exercise were likely due to hemoconcentration from insensible water loss, splenic contraction and sympathetic stimulation while 4-hours later autohemodilution reduced RBC parameters. Elevations in plasma lactate and urinary TXB2 correlated with advanced skill level/speed of the dogs.

Keywords:
Agility exercise; Dog; Urinary isoprostane; Plasma lactate; Oxidant stress; Urinary eicosanoids; Thromboxane B2