Passage of doxycycline (a) and paromomycin (b) across a differentiated IPEC-J2 cell monolayer, measured as the concentration in the basolateral compartment. Cells were grown for 21 days on Transwell® collagen-coated PTFE filters (0.33 cm2, 0.4 μm). Next, at day 0, cells were incubated for 72 h with different concentrations of DON (untreated inserts, inserts treated with 0.5, 1, 5 or 10 μg/ml), during which the last 24 h a non-cytotoxic concentration of doxycycline and paromomycin was added to the apical compartment (n = 3, mean + stdev). Significant differences in passage have been marked with * (P < 0.05), ** (P < 0.01) or *** (P < 0.001) according to the significance level.
Goossens et al. BMC Veterinary Research 2012 8:245 doi:10.1186/1746-6148-8-245