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Open Access Highly Accessed Research article

The diversity of the fecal bacterial community and its relationship with the concentration of volatile fatty acids in the feces during subacute rumen acidosis in dairy cows

Shengyong Mao*, Ruiyang Zhang, Dongsheng Wang and Weiyun Zhu

Author Affiliations

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

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BMC Veterinary Research 2012, 8:237  doi:10.1186/1746-6148-8-237

Published: 6 December 2012

Additional files

Additional file 1: Figure S1:

Influence of SAID feeding on fecal microbiota of dairy cattle at the level of bacterial class. A. Distribution of all bacterial classes among diets and animals as revealed by heatmap. B. The bacterial classes for which abundance was significantly affected by the diet. Legend as for Figure 2.

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Additional file 2: Figure S2:

Influence of SAID feeding on fecal microbiota of dairy cattle at the level of bacterial order. A. Distribution of all bacterial orders among diets and animals. B. The bacterial orders for which abundance was significantly affected by the diet. Legend as for Figure 2.

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Additional file 3: Figure S3:

Influence of SAID feeding on fecal microbiota of dairy cattle at the level of bacterial family. A. Distribution of the top (≥ 99.8% abundant) families observed among dietary treatments. B. The bacterial families for which abundance was significantly affected by the diet. Legend as for Figure 2.

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Additional file 4: Figure S4:

Venn diagram of the overlap between observed OTUs at 3% divergence in COD and SAID groups. The number of OTUs found exclusively in COD was 1349 and in SAID samples was 1207. The number of OTUs shared between COD and SAID was 440. The percentage of shared OTUs was 21%. Data are also represented by the phylum to which the detected OTUs belong. Data regarding genera are shown as supplementary material.

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Additional file 5: Table S1:

The OTUs at the genus level in COD and SAID groups.

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Additional file 6: Table S2:

The changes in fecal microbial composition at the species level. Only the species that were significantly affected in percentage by the type of diet are presented.

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Additional file 7: Table S3:

Diet composition and ingredients of experimental diets.

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