Table 2

The percentage of OTUs that were assigned to the 20 most abundant microbial genera
Bacterial genus assignment Percent of all horses with genus present Pooled Control group Laminitis group
Mean Mean S.E. Mean S.E.
N = 13 N = 7 N = 5
Streptococcus 100% 21.00% 24.10% 9.03% 16.03% 8.71%
RFP12 (O) 100% 19.96% 16.79% 5.32% 25.02% 4.01%
Ruminococcaceae (F) 100% 8.95% 8.30% 1.92% 9.99% 1.54%
Clostridiaceae (F) 100% 6.20% 10.03%* 0.50% 0.07% 1.29%
Bacteroidales (O) 100% 5.41% 3.96% 0.64% 7.73% 0.67%
Clostridium 100% 4.75% 5.30% 2.87% 3.88% 0.80%
Clostridiales (O)* 100% 4.58% 3.80% 1.03% 5.82% 0.92%
Lachnospiraceae (F) 100% 4.14% 4.07% 1.53% 4.25% 0.90%
Treponema 100% 2.58% 2.87% 0.34% 2.10% 0.34%
Clostridiales (O)* 100% 2.27% 1.63% 0.34% 3.29% 0.39%
Ruminococcaceae (F) 100% 1.81% 1.66% 0.60% 2.04% 0.26%
Akkermansia 94% 1.80% 1.30% 0.32% 2.61% 0.56%
Oscillospira 100% 1.44% 1.51% 0.48% 1.34% 0.13%
Ruminococcus 88%% 1.30% 1.53% 0.49% 0.95% 0.22%
Lachnospiraceae (F) 100% 1.16% 1.02% 0.27% 1.39% 0.15%
Clostridiales XIII I. sedis (F) 100% 0.99% 0.50% 0.17% 1.78% 0.13%
Firmicutes (P) 94% 0.93% 0.81% 0.71% 1.12% 0.30%
Lactobacillus 88% 0.82% 0.88% 0.05% 0.72% 0.18%
Staphylococcus 67% 0.78% 1.27% 0.57% 0.00% 0.00%
Coprococcus 88% 0.65% 0.60% 0.27% 0.73% 0.21%

The OTU assignments were made using the Ribosomal Database Project classifier in QIIME. The mean and standard error (S.E.) of the percentage of reads that map to the respective genus is provided. If the OTU was not assigned to a known genus its nearest available taxonomic rank is provided. F = Family, O = Order, P = Phylum. *Significant differences between groups (P = 0.03 and 0.01, respectively).

Steelman et al.

Steelman et al. BMC Veterinary Research 2012 8:231   doi:10.1186/1746-6148-8-231

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