Table 1

Diversity indices of the gastrointestinal microflora in horses
All horses Control Laminitis
97% Similarity OTUs
 OTUs per animal 381.7 (SE = 26.6) 355.2 (SE = 26.3) 419.6 (SE = 49.3)
 OTUs (rfd)* 338.8.0 (SE = 52.3) 330.4 (SE = 57.5) 369.5 (SE = 51.8)
 Chao1 795.7 (SE = 51.7) 741.9 (SE = 66.2) 872.6 (SE = 77.4)
 Chao1 (rfd)* 761.3 (SE = 82.2) 735.7 (SE = 123.4) 816.4 (SE = 65.5)
 Phylogenetic Distance 16.15 (SE = 0.82) 15.37 (SE = 0.85) 17.26 (SE = 1.50)
 Phylogenetic Distance (rfd) 14.85 (SE = 1.50) 14.43 (SE = 1.05) 16.00 (SE = 2.00)
 Shannon 5.07 (SE = 0.59) 5.01 (SE = 0.71) 5.16 (SE = 1.06)
 Simpson 0.74 (SE = 0.08) 0.75 (SE = 0.09) 0.72 (SE = 0.14)
Genera
 Total 108 101 89
 Mean per animal 53.4 (SE ± 1.60) 52.3 (SE ± 1.60) 55.0 (SE ± 1.65)

The OTUs (97% similarity), Chao1, Phylogenetic Distance, Shannon, and Simpson diversity indices were estimated in QIIME. Rarified OTU, Chao1, and Phylogenetic Distance estimates were rarefied (rfd) to a depth of 1,100 reads to reduce sampling heterogeneity. * Significant difference, (OTUs, P = 0.019; Chao1, P = 0.020).

Steelman et al.

Steelman et al. BMC Veterinary Research 2012 8:231   doi:10.1186/1746-6148-8-231

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