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Pyrosequencing of 16S rRNA genes in fecal samples reveals high diversity of hindgut microflora in horses and potential links to chronic laminitis

Samantha M Steelman1, Bhanu P Chowdhary1, Scot Dowd2, Jan Suchodolski3 and Jan E Janečka1*

Author Affiliations

1 Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, 77843-4458, USA

2 Molecular Research LP, Shallowater, 79363, TX, USA

3 Gastrointestinal Laboratory, Texas A&M University, College Station, TX, 77843-4458, USA

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BMC Veterinary Research 2012, 8:231  doi:10.1186/1746-6148-8-231

Published: 27 November 2012

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Additional file 1:

Table S1. Description of horses in each study group. The exact age of all horses in the laminitis group was not known; one horse was estimated to be 7 years of age, the others were over 15 years. None of the horses with laminitis exhibited signs of Cushing’s syndrome, although the initial cause of laminitis was not known for all animals. Abbreviations are as follows: BCS – body condition score [65], QH – Quarter horse, WB – Warmblood, TB – Thoroughbred. Table S2 Feed analysis. Comparison of guaranteed feed analysis of 12% concentrate pellets and 16% concentrate pellets used in this study. Pellets were formulated by Producer’s Co-op (Bryan, TX) and further information is available online: webcite.

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