Western blot analysis of cross reaction of the NA1/11 strain with ORFV-positive antiserum and monoclonal antibodies A3 against ORFV-Jilin ORFV059. 2 μg of the purified viral protein or 20 μg of OFTu total cell lysate were resolved by using SDS-PAGE, transferred to nitrocellulose paper, probed with infected sheep serum (A) and Mab A3 against ORFV-Jilin ORFV059 (B). The strongest reactive protein bands were 39, 25, 20 and 15 kDa but 28, 35, 49, 55, 60 and 90 kDa bands were detectable (A, Lane 2) The location of an approximately 39 kDa protein corresponding in molecular weight to ORFV059 protein is shown (B, Lane 2). The lane 1 of A and B is protein marker.
Li et al. BMC Veterinary Research 2012 8:229 doi:10.1186/1746-6148-8-229