Immunofluorescence of canine myotubes.A, C: immunofluorescence staining of differentiated, multinucleated canine myotubes positive for muscle-specific intermediate filament desmin, primary antibody: mouse anti-Desmin, secondary antibody: goat anti-mouse IgG, Cy3 labelled. B, D: 4′,6-Diamidino-2-phenylindol nucleus staining (=DAPI) demonstrating multiple nuclei of the myotubes (in 60–80% of the cells). E, F: canine fibrocytes culture as negative control, (A, B 400-fold magnification; C, D, E, F 200-fold magnification; bar = 100 μm).
Schenk et al. BMC Veterinary Research 2012 8:227 doi:10.1186/1746-6148-8-227