Table 2

PMCA-amplification of infected flock/farm mates of ARQ/ARQ scrapie-infected sheep
Farm* Sheep ID Breed Age at death PMCA substrates#
VRQ/VRQ ARQ/ARQ
12 913/05 Mule 5y 0 m + +
SS0-560272 Suffolk Cross 4y + +
15 1230/04 Suffolk Cross 2y 4 m + +
709/02 Cheviot/Shetland 5y + +
768/02 Cheviot/Shetland 4y + +
857/04 Cheviot/Shetland 7y 3 m + +
858/04 Cheviot/Shetland 3y 3 m + +
872/04 Cheviot/Shetland 7y 3 m + +
13 960/06 White Face Dartmoor 7y + +
703/07 White Face Dartmoor 6y + +
912/07 White Face Dartmoor 7y + +
11 297/97 Finn Dorset not known - +
210/03 Warborough 2y 2 m + +
209/03 Warborough 5y 1 m - +
1583/97 Finn Dorset 4y - +
1685/97 Finn Dorset 4y - +
1002/03 Finn Dorset Cross 7y - +
1158/00 Finn Dorset 6y - +
57/99 Finn Dorset 5y 1 m - +

*Farms 12, 15, 13 and 11 were located in Gloucestershire, Aberdeenshire, Devon and Oxfordshire respectively. Samples were analysed after 4 rounds of PMCA.

#Each sample was amplified twice using a single substrate of each genotype and a positive sample was recorded when positive in at least one replicate.

Thorne et al.

Thorne et al. BMC Veterinary Research 2012 8:223   doi:10.1186/1746-6148-8-223

Open Data