Table 1

In vitro PMCA amplification of sources of GB scrapie
Sheep ID PrnP genotype Farm Geographic Location* Age at death Amplification in substrates
VRQ ARQ
1597/98 VRQ/VRQ 1 E. England 2y 7 m + -
322/97 2 Wales 2y 0 m + -
1563/02 3 W. England 2y 8 m + -
836/03 4 S. England 2y 2 m + -
1276/02 5 Wales 2y 0 m + ND#
2413/98 6 W. England 2y 2 m + ND
456/03 ARQ/VRQ 7 N. England 3y 0 m + -
455/03 7 N. England 6y 0 m + -
615/03 7 N. England 5 y 0 m + -
226/03 7 N. England 5y 0 m + -
ss2-022278 8 W. England not known + -
219/06 ARH/VRQ 17 W. England 2y 0 m + +
252/06 17 W. England 2y 0 m + +
198/06 17 W. England 2y 0 m + +
216/06 17 W. England 2y 0 m + +
989/02 ARR/VRQ 9 S. England 8y 0 m + ND
ss4 506045 10 N. England not known + -
ss4-506228 not known not known not known + -
297/97 ARQ/ARQ 11 S. England not known - +
ss4-012265 10 N. England not known - +
913/05 12 W. England 5y 0 m + +
522 not known not known not known ND +
960/06 13 W. England 7y 0 m + +
1341/04 14 E. England 5y 5 m - +
1230/04 15 Scotland 2y 4 m + +
284/97 AHQ/AHQ 11 S. England not known - +
1091/01 16 Midlands 4y 8 m - +
217/06 ARH/ARH 17 W. England 1y 0 m + +
429/06 17 W. England 2y 0 m + +
1731/03 ARH/ARQ 18 N. England 1y 6 m + +
1741/03 18 N. England 1y 2 m + +
1952/04 19 W. England 3y 0 m + +
936/05 20 Wales 4y 0 m + +
708/02 15 Scotland 5y 0 m + +
399/04 15 Scotland 3y 0 m + +
462/04 15 Scotland 2y 0 m + +
915/07 13 S. England 1y 6 m + +

*S. England: South of England, N. England: North of England, W. England: Welsh borders and West Country, E. England: East of England. All samples were analysed in duplicate after four rounds of PMCA and those marked + were positive in at least one replicate. #ND, not done.

Thorne et al.

Thorne et al. BMC Veterinary Research 2012 8:223   doi:10.1186/1746-6148-8-223

Open Data