MRI Suffolk and CH1641 scrapie amplified in the presence of poly(A) in substrates with different PRNP genotypes. Unamplified samples (lanes 1) or amplified (0.3 mg wet tissue equivalent loaded per lane) in VRQ/VRQ substrate (lanes 2), ARQ/ARQ substrate (lanes 3 and 4) or AHQ/AHQ (lanes 5) in the presence of Poly(A). Lane 6 contains products amplified in AHQ/AHQ substrate in the absence of Poly(A). All amplifications were analysed after 4 rounds. Molecular mass markers are indicated (kDa). Upper panels are visualised with Sha31 and lower panels with P4 antibodies.
Thorne et al. BMC Veterinary Research 2012 8:223 doi:10.1186/1746-6148-8-223